| ORGANOLEPTIC TEST | | |
| Appearance, color, taste, smell, structure, appearance, consistency | BDS 8142, item1.3, таб.1, item3.1; BDS 483, item2.3.1, таб.1, item4.1, item4.2; BDS 2306, item4.1; BDS 5757, item4.1 и item 4.2; BDS 2608, item4.2; BDS 10945, item 3.2.1 | |
| PHYSICO-CHEMICAL AND BIOCHEMICAL TESTS | | |
| Active acidity (рН) | QOP 504-1-FH-26 | |
| Titrable (free acidity) | BDS 8142 item3.2; BDS 483 item4.5; QOP 504-1-FH-12 | |
| Water content/dry matter | BDS EN ISO 712; BDS 16752; BDS 483 item4.3 | |
| Fat content (crude fat) - extractive | QOP 504-1-FH-42 | |
| Protein content (protein, nitrogen) | BDS ISO 1871 | |
| Sugars content (sucrose, lactose, reducing sugars/invert sugar) | BDS 7169 | |
| Carbohydrates content | QOP 504-1-FH-41 | |
| Starch content | Regulation 900/2008 Annex I | |
| Crude cellulose content (fibers) | BDS ISO 5498; BDS ISO 6541 | |
| Ash content | BDS EN ISO 2171; BDS 8142 item3.3 | |
| Table salt content /sodium chloride | QOP 504-1-FH-03 | |
| Energy value | чл. 10 от Наредба №23/2009 | |
| Content of ascorbic acid (Vitamin C) | QOP 504-1-FH-45 | |
| Sodium hydroxide residues content | QOP 504-1-FH-15 | |
| Chlorine residues content | QOP 504-1-FH-27 | |
| Testing for presence of epihydrin aldehyde through Kreis reaction (Fat rancidity) | QOP 504-1-FH-30 | |
| Starch content – quality response | QOP 504-1-FH-28 | |
| Determination of peroxide value | BDS EN ISO 3960 | |
| Benzoic acid content | BDS 1554 item III.15 | |
| Potassium sorbate content | BDS 11712 item2 | |
| Sulfur dioxide content | BDS 11709 item1.2;item2 | |
| Soy protein content | QOP 504-1-FH-32 | |
| Aluminum content | QOP 504-1-FH-07 | |
| Antimony content | QOP 504-1 ICP/MS-04 | |
| Arsenic content | QOP 504-1-FH-19; BDS EN 15763 | |
| Barium ions presence | BDS 8142 item3.8 | |
| Boron content | BDS EN ISO 17294-2 | |
| Iron content | QOP 504-1-FH-02 | |
| Mercury content | BDS EN 15763 | |
| Iodine content | QOP 504-1-FH-46 | |
| Cadmium content | QOP 504-1-FH-17; BDS EN 15763 | |
| Tin content | BDS EN 15765 | |
| Calcium content | QOP 504-1-FH-21 | |
| Magnesium content | QOP 504-1-FH-52 | |
| Manganese content | QOP 504-1-FH-04 | |
| Copper content | QOP 504-1-FH-18 | |
| Sodium content | QOP 504-1 ICP/MS-02 | |
| Nickel content | QOP 504-1-FH-24 | |
| Nitrates content | QOP 504-1-FH-08 | |
| Nitrites content | QOP 504-1-FH-05 | |
| Lead content | QOP 504-1-FH-16; BDS EN 15763 | |
| Selenium content | BDS EN ISO 17294-2 | |
| Sulphates content | QOP 504-1-FH-09 | |
| Phosphates (phosphorus) content | QOP 504-1-FH-06 | |
| Chromium content | QOP 504-1-FH-25 | |
| Cyanides content | QOP 504-1-FH-53 | |
| Ferrocyanides content in citric acid( | BDS 8142 item3.9 | |
| Zinc content | QOP 504-1-FH-23 | |
| Aflatoxin В1 content | QOP 504-1-FH-20 | |
| Aflatoxines В1+В2+G1+G2 (total) | QOP 504-1-FH-34 | |
| Aflatoxin M1 content | QOP 504-1-FH-31 | |
| Deoxynivalenol /DON/ content | QOP 504-1-FH-35 | |
| Zearalenone content | QOP 504-1-FH-36 | |
| Ochratoxin content | QOP 504-1-FH-38 | |
| Т2 toxin content | QOP 504-1-FH-39 | |
| Fumonisin content | QOP 504-1-FH-37 | |
| Ethanol (ethyl alcohol) content | BDS ISO 2448 | |
| Connective tissue protein (hydroxyproline) content | BDS 14780 | |
| Gluten content | BDS EN ISO 21415-3 | |
| Saccharine content | BDS 3485 item3.7 | |
| Caffeine content | BDS 3485 item3.14 | |
| Free sulphuric acid content | BDS 8142 item3.4.1 | |
| Oxalates content | BDS 8142 item3.7 | |
| Buoyant force of bread yeast | BDS 483 item4.4 | |
| Urease activity | BDS ISO 5506 | |
| Potassium content | QOP 504-1 ICP/MS-01 | |
| Content of pesticide: Chlororganic Phosphororganic Nitrogen and sulfur-containing | BDS EN ISO 15662 | |
| Sweeteners content: | | |
| - acesulfame К | BDS EN 12856; BDS EN 15911 | |
| - saccharine | BDS EN 12856; BDS EN 15911 | |
| - aspartame | BDS EN 12856; BDS EN 15911 | |
| - cyclamate | BDS EN 12857; BDS EN 15911 | |
| Colorings content: | | |
| Yellow: | | |
| - Sunset Yellow | QOP 504-1-IM-HPLC-01 | |
| - tartrazine | QOP 504-1-IM-HPLC-01 | |
| Red: | | |
| - erythrosine | QOP 504-1-IM-HPLC-01 | |
| - azorubine | QOP 504-1-IM-HPLC-01 | |
| - allura red | QOP 504-1-IM-HPLC-01 | |
| - ponceau | QOP 504-1-IM-HPLC-01 | |
| - amaranth | QOP 504-1-IM-HPLC-01 | |
| Blue | | |
| - indigotine | QOP 504-1-IM-HPLC-01 | |
| - Brillian Blue | QOP 504-1-IM-HPLC-01 | |
| - Patent Blue | QOP 504-1-IM-HPLC-01 | |
| Vitamin content: | | |
| - А (retinol) | BDS EN 12823-1;2 | |
| - А (beta-carotine) | BDS EN 12823-1;2 | |
| - В1 (thiamine) | QOP 504-1-IM-LC/MS-02 | |
| - В3 (niacin) | QOP 504-1-IM-LC/MS-02 | |
| - В6 (pyridoxine) | QOP 504-1-IM-LC/MS-02 | |
| - В7 (biotin/vitamin Н) | QOP 504-1-IM-LC/MS-02 | |
| - С (ascorbic acid) | BDS EN 14130 | |
| - D2 ergocalciferol | BDS EN 12821 | |
| - D3 colecalciferol | BDS EN 12821 | |
| - Е (tocopherols) | BDS EN 12822 | |
| - К (phytomenadione) | BDS EN 14148 | |
| Content of saturated fatty acids | BDS EN ISO 12966-2+BDS EN ISO 5508 | |
| Content of unsaturated fatty acids | BDS EN ISO 12966-2+BDS EN ISO 5508 | |
| Pharmacological substances residues content: | | |
| Antibiotics (Quinolines) – flumequin | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfaguanidin | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfadiazine | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfamerazin | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfamethoxazole | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfapyridine | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfatiazol | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Sulfonamides) - sulfacetamide | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Tetracyclines) – oxytetracycline хидрохлорид | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Tetracyclines) – tetracycline hydrochloride | QOP 504-1-IM-LC/MS-01 | |
| Antibiotics (Tetracyclines) – chlortetracycline hydrochloride | QOP 504-1-IM-LC/MS-01 | |
| MICROBIOLOGICAL TESTS | | |
| Total number of microorganisms, including mesophilic aerobes and facultative anaerobes | BDS ЕN ISO 4833-1 BDS ЕN ISO 4833-2 | |
| Antibiotics and Sulfonamides | BDS 1323 item4.3.1 | |
| Bacillus Cereus | BDS EN ISO 21871 item9.; item9.2; BDS EN ISO 7932 | |
| Bifidobacterium species | ISO 29981 | |
| Brochothrix thermosphacta | BDS ISO 13722 | |
| Vibrios (other than Vibrio parahaemolyticus and Vibrio cholerae) | ISO/TS 21872-2 | |
| Water activity | ISO 21807 | |
| Yeast | BDS ISO 21527-1; -2 | |
| Yeast (including dead) | BDS 483 item4.7 | |
| Yeast (including wild) | BDS 483 item4.8 | |
| Escherichia coli | ISO 16649-2; ISO 7251 item9.1; item9.2 | |
| Escherichia coli O157 | BDS EN ISO 16654 | |
| Enterobacteria, including Proteus, Klebsiella, Citrobacter | ISO 21528-1 item9.2; item9.3; ISO 21528-2 | |
| Enterococus species | BDS EN 15788 | |
| Yersinia enterocolitica | BDS EN ISO 10273 | |
| Campylobacter spp. | ISO 10272-1; BDS ISO/TS 10272-2 | |
| Clostridia Sulfite-reducing | ISO 15213 | |
| Coagulase-positive staphylococci | BDS EN ISO 6888-1+A1; -2+A1; -3+AC item9.1; item9.2 | |
| Coliforms | ISO 4831item9.1; item9.2; ISO 4832 | |
| Lactobacillus species | BDS ISO 7889 | |
| Lactobacillus acidophilus | ISO 20128 | |
| Lactobacillus delbrueckii subsp. Bulgaricus | BDS ISO 9232 | |
| Leuconostoc | COMECON ST 5806 | |
| Lipolytic microorganisms | BDS 12334 item7 | |
| Listeria monocytogenes | BDS EN ISO 11290-1+А1; -2+А1 | |
| Mesophilic lactic bacteria | BDS ISO 15214 | |
| Acetobacter microorganisms | BDS 15143 item5.1 | |
| Pediococcus species | BDS EN 15786 | |
| Moulds | BDS ISO 21527-1; -2 | |
| Probiotics (saccharomyces cerevisiae) | BDS EN 15789 | |
| Proteolytic microorganisms | BDS 3434 item10.3 | |
| Pseudomonas aeruginosa | BDS EN ISO 13720 | |
| Psychrotrophic microorganisms | BDS ISO 17410 | |
| Salmonella species | BDS ЕN ISO 6579; SD СЕN ISO/TS 6579-2 | |
| Streptococcus thermophilus | BDS ISO 9232 | |
| Halophilic (salt-tolerant) microorganisms | BDS 3434 item10.4 | |
| Shigella species | BDS EN ISO 21567 | |
| Contaminating microorganisms | ISO 13559 | |
| Genetically modified organisms | | |
| Determination of presence of genetically modified organisms – 35S | BDS EN ISO 21569 +A1; BDS EN ISO 21571 +A1 | |
| Determination of presence of genetically modified organisms – FMV | BDS EN ISO 21569 +A1; BDS EN ISO 21571 +A1 | |
| Determination of presence of genetically modified organisms – NOS terminator | BDS EN ISO 21569 +A1; BDS EN ISO 21571 +A1 | |